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Volume 1, Issue 1, Agricultural Science and Food Processing
Volume 1, Issue 1, 2024
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Jie Ouyang
Jie Ouyang
Beijing Forestry University, China
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Agricultural Science and Food Processing, Volume 1, Issue 1, 2024: 29-37

Free to Read | Research Article | 10 November 2024
Evaluation of the Compound-microbes-preparation Based on Microflora Information as Inoculants in the Silage
1 Academy of State Administration of Grain, Beijing 100037, China
* Corresponding Author: Wei Han, [email protected]
Received: 19 September 2024, Accepted: 21 October 2024, Published: 10 November 2024  
Abstract
Ensiling technique is becoming a common way to preserve fresh corn and grass. This study aimed at evaluating the silages’ quality with a new type of inoculants designed based on the microflora information of the native silages. A total of 33 silage samples were analyzed for their microbial community structure by high throughput sequencing. A total of 73 dominant strains were isolated with a clear goal. The corn was ensiled by dominant strains (Lactobacillus buchneri II JC1-5: Acetobacter pasteurianus DHL-8 = 4:3; 109 CFU/g) as inoculants. In a 15-day trial period, the aerobic stability of this inoculated silage was significantly higher than the control group. There was a positive correlation between lactic acid content and pH/mold number (R2 values of 0.64331 and 0.48584, respectively). Compared to the control group, ammonia nitrogen (NH3-N), water soluble carbohydrates (WSC), neutral detergent fiber (NDF), and acid detergent fiber (ADF) decreased by 57.7%, 12.5%, 1.4%, and 7.5%, respectively; crude protein (CP) increased by 1.1%. Our results indicate that the new type of inoculants can improve the quality of the silage.

Graphical Abstract
Evaluation of the Compound-microbes-preparation Based on Microflora Information as Inoculants in the Silage

Keywords
silage
inoculants
microflora
high throughput sequencing

Funding
This work was supported without any funding.

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Cite This Article
APA Style
Zhuang, X., Han, W., Xue, Z., & Miao, H. (2024). Evaluation of the Compound-microbes-preparation Based on Microflora Information as Inoculants in the Silage. Agricultural Science and Food Processing, 1(1), 29–37. https://doi.org/10.62762/ASFP.2024.516036

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